Biocompatability of hydroxyapatite composite as a local drug delivery system

A Krisanapiboon, B Buranapanitkit
Department of Orthopaedic and Physical Medicine, Faculty of Medicine, Prince of Songkla University, Haadyai, Songkhla, Thailand

K Oungbho
Department of Pharmaceutical Technology, Faculty of Pharmaceutical Science, Prince of Songkla University, Haadyai, Songkhla, Thailand

Purpose. To investigate the biocompatibility of hydroxyapatite composite (hydroxyapatite, plaster of Paris, and chitosan) impregnated with gentamicin, fosfomycin, imipenem, or amphotericin B.

Methods. The interactions of the extract from each drug against osteoblast were tested using the methylthiotetrazole test.

Results. Extracts from all drugs showed good biocompatibility at concentrations varying from 10 £gg/ml to 1000 £gg/ml. Imipenem and amphotericin B at a concentration of 1000 £gg/ml had a significantly higher percentage of cell viability than the control group. No morphological change of osteoblast was observed in all drug tests at any concentrations.

Conclusion. The hydroxyapatite composite had a good biocompatibility for carrying gentamicin, fosfomycin, imipenem, or amphotericin B.